The reagent is intended for use as quality control in platelet function testing with whole blood samples on the Multiplate® analyzer.
The reagent is employed in combination with the Multiplate®activating reagent TRAPtest. Addition of GpIIb/IIIa Antagonist Reagent to a blood sample leads to strongly reduced aggregation in the TRAPtest and diminished aggregation in Multiplate® tests. This allows the assessment of a positive control (strongly inhibited aggregation).
In vitro platelet aggregation by electrical impedance relies on recruitment of platelets to a surface (electrode wire) by means of platelet-fibrinogen bonds in order to measure a response. The introduction of a substance that blocks the binding of fibrinogen to the resting or activated GPIIb/IIIa receptor will result in reduced or absent platelet aggregation to all agonists1,2 including TRAP-6.
Multiplate® ASA Reagent
The ASA Reagent (acetylsalicylic acid) is intended for use as quality control in platelet aggregation function testing with whole blood samples on the Multiplate® analyzer. Addition of ASA Reagent to the blood sample leads to reduced aggregation responses in ASPItest and COLtest.
Upon addition to the blood sample the platelet cyclooxygenase pathway is blocked and the cyclooxygenase-dependent Multiplate® tests, ASPItest and COLtest are inhibited. This allows the assessment of an abnormal response in these tests.
The Multiplate® Liquid Control set contains two solutions (Solution 1 (2 x 4 ml) and Solution 2 (1 x 2 ml)) of different ionic strengths. The Liquid Control Set is used as an assayed quality control material to verify resistance measurements of the analyzer. The set contains sufficient volume to test the Level 1 and Level 2 control in all channels if required.